Voskarides K1,2, Papagregoriou G3, Hadjipanagi D3, Petrou I3, Savva I3, Elia A4, Athanasiou Y5, Pastelli A6, Kkolou M6, Hadjigavriel M6, Stavrou C7, Pierides A3,8, Deltas C9,10.
1 Molecular Medicine Research Center, Department of Biological Sciences, University of Cyprus, 1, University Avenue, 2109, Nicosia, Cyprus. email@example.com.
2 Medical School, University of Cyprus, Nicosia, Cyprus. firstname.lastname@example.org.
3 Molecular Medicine Research Center, Department of Biological Sciences, University of Cyprus, 1, University Avenue, 2109, Nicosia, Cyprus.
4 Department of Pediatric Nephrology, Archbishop Makarios III Hospital, Nicosia, Cyprus.
5 Department of Nephrology, Nicosia General Hospital, Nicosia, Cyprus.
6 Department of Nephrology, Larnaca General Hospital, Larnaca, Cyprus.
7 Department of Nephrology, Evangelismos Hospital, Pafos, Cyprus.
8 Hippocrateon Hospital, Nicosia, Cyprus.
9 Molecular Medicine Research Center, Department of Biological Sciences, University of Cyprus, 1, University Avenue, 2109, Nicosia, Cyprus. email@example.com.
10 College of Medicine, Qatar University, Doha, Qatar. firstname.lastname@example.org.
About 40-50% of patients with familial microscopic hematuria (FMH) caused by thin basement membrane nephropathy (TBMN) inherit heterozygous mutations in collagen IV genes (COL4A3, COL4A4). On long follow-up, the full phenotypic spectrum of these patients varies a lot, ranging from isolated MH or MH plus low-grade proteinuria to chronic renal failure of variable degree, including end-stage renal disease (ESRD).
Here, we performed Whole Exome Sequencing (WES) in patients of six families, presenting with autosomal dominant FMH, with or without progression to proteinuria and loss of renal function, all previously found negative for severe collagen IV mutations. Hierarchical filtering of the WES data was performed, followed by mutation prediction analysis, Sanger sequencing and genetic segregation analysis.
In one family with four patients, we found evidence for the contribution of two co-inherited variants in two crucial genes expressed in the glomerular basement membrane (GBM); LAMA5-p.Pro1243Leu and COL4A5-p.Asp654Tyr. Mutations in COL4A5 cause classical X-linked Alport Syndrome, while rare mutations in the LAMA5 have been reported in patients with focal segmental glomerulosclerosis. The phenotypic spectrum of the patients includes hematuria, proteinuria, focal segmental glomerulosclerosis, loss of kidney function and renal cortical cysts.
A modifier role of LAMA5 on the background of a hypomorphic Alport syndrome causing mutation is a possible explanation of our findings. Digenic inheritance is another scenario, following the concept that mutations at both loci more accurately explain the spectrum of symptoms, but further investigation is needed under this concept. This is the third report linking a LAMA5 variant with human renal disease and expanding the spectrum of genes involved in glomerular pathologies accompanied by familial hematurias. The cystic phenotype overlaps with that of a mouse model, which carried a Lama5 hypomorphic mutation that caused severely reduced Lama5 protein levels and produced kidney cysts.