Read more about the article Membrane adsorption with direct cell culture combined with reverse transcription-PCR as a fast method for identifying enteroviruses from sewage.
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Membrane adsorption with direct cell culture combined with reverse transcription-PCR as a fast method for identifying enteroviruses from sewage.

Abstract We present a new approach for the detection and identification of enteroviruses concentrated and isolated from sewage. Samples were collected from two study sites located at Nicosia and Limassol…

Continue ReadingMembrane adsorption with direct cell culture combined with reverse transcription-PCR as a fast method for identifying enteroviruses from sewage.
Read more about the article Engineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection.
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Engineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection.

Abstract A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes…

Continue ReadingEngineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection.
Read more about the article Differentiation of Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus using real-time TaqMan PCR.
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Differentiation of Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus using real-time TaqMan PCR.

Abstract During the past four decades, Tomato yellow leaf curl disease has become one of the major constraints in tomato production worldwide. In the Mediterranean basin, several isolates from two…

Continue ReadingDifferentiation of Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus using real-time TaqMan PCR.
Read more about the article Fetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21.
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Fetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21.

Abstract The trials performed worldwide toward noninvasive prenatal diagnosis (NIPD) of Down's syndrome (or trisomy 21) have shown the commercial and medical potential of NIPD compared to the currently used…

Continue ReadingFetal-specific DNA methylation ratio permits noninvasive prenatal diagnosis of trisomy 21.
Read more about the article Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.
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Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.

Abstract Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need…

Continue ReadingDevelopment of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.
Read more about the article Technical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds.
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Technical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds.

Abstract In the present study, a rapid and cost-effective PCR-based assay was developed for the genetic identification of 2 different variants within intron 2 of the prolactin gene. This polymorphism…

Continue ReadingTechnical note: A simplified PCR-based assay for the characterization of two prolactin variants that affect milk traits in sheep breeds.
Read more about the article Rapid discrimination of Tomato chlorosis virus, Tomato infectious chlorosis virus and co-amplification of plant internal control using real-time RT-PCR.
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Rapid discrimination of Tomato chlorosis virus, Tomato infectious chlorosis virus and co-amplification of plant internal control using real-time RT-PCR.

Abstract Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) (genus: Crinivirus, family: Closteroviridae) are two emergent whitefly-transmitted viruses that have been associated with yellowing symptoms of tomato crops…

Continue ReadingRapid discrimination of Tomato chlorosis virus, Tomato infectious chlorosis virus and co-amplification of plant internal control using real-time RT-PCR.
Read more about the article Diagnostic real-time RT-PCR for the simultaneous detection of Citrus exocortis viroid and Hop stunt viroid.
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Diagnostic real-time RT-PCR for the simultaneous detection of Citrus exocortis viroid and Hop stunt viroid.

Abstract Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are two important viroids known to infect several plant species worldwide. In this study, a real-time reverse transcription (RT) TaqMan…

Continue ReadingDiagnostic real-time RT-PCR for the simultaneous detection of Citrus exocortis viroid and Hop stunt viroid.
Read more about the article Calreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring.
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Calreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring.

Abstract The diagnosis of the BCR-ABL-negative myeloproliferative neoplasms (MPN), namely polycythemia vera, essential thombocythemia and primary myelofibrosis has relied significantly on the detection of known causative mutations in the JAK2…

Continue ReadingCalreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring.
Read more about the article Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?
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Is "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?

Abstract PCR and DNA sequencing are currently the diagnostic methods of choice for detection of Blastocystis spp. and their suptypes. Fresh or frozen stool samples have disadvantages in terms of…

Continue ReadingIs "dried stool spots on filter paper method (DSSFP)" more sensitive and effective for detecting Blastocystis spp. and their subtypes by PCR and sequencing?